Part:BBa_K3889012
Bacillus subtilis Synthetic Promoter 200
This is a synthetically derived promoter sequence for constitutive expression of genes in Bacillus subtilis.
| Promoter | Sequence 5‘ → 3‘ | Relative activity wrt P43 of GFP (%) | Standard deviation |
|---|---|---|---|
| SP126 | AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT | 29.07 | 0.23 |
| SP146 | AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT | 40.39 | 0.69 |
| SP200 | AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA | 76.82 | 0.9 |
Synthetic Promoters data from SP1-214[1]
Usage and Biology
SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889012) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. The library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
Based on this the promoters are as follows:
Fig source: Geneious version 2021.2 created by Biomatters. Available from https://www.geneious.com
Modelling
We planned to use SP200 with BBa_K1351031 for gene expression of P22(BBa_K3889101).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115
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